9DCF

Structure of Coxsackievirus B3 cloverleaf RNA in complex with 3Cpro dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 2
Unit cell lengths	91.018, 200.821, 71.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 2.870
R-factor	0.2394
Rwork	0.237
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.282
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.21.1_5286)
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.930	2.950
High resolution limit [Å]	2.870	2.870
Rmeas	0.337
Rpim	0.098	0.833
Number of reflections	29950	1384
<I/σ(I)>	5	1
Completeness [%]	98.8
Redundancy	11
CC(1/2)	0.947	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	8% v/v Tacsimate, pH 6.0, 25% w/v PEG3350