9DC0

Crystal Structure of European Robin CRY1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	44.115, 79.602, 127.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.590 - 1.910
R-factor	0.2127
Rwork	0.209
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.590	1.974
High resolution limit [Å]	1.910	1.910
Rmerge	0.133	1.315
Number of reflections	35451	2929
<I/σ(I)>	9.2	0.84
Completeness [%]	98.7
Redundancy	5.9
CC(1/2)	0.996	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	8-10% PEG 8000 0.1 M Hopes pH 7.5