9DBH

Structure of Hailong HalB in complex with oligodeoxyadenylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-08-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 1 21 1
Unit cell lengths	56.365, 133.269, 79.165
Unit cell angles	90.00, 107.92, 90.00

Refinement procedure

Resolution	37.660 - 1.880
R-factor	0.1966
Rwork	0.196
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.330	1.990
High resolution limit [Å]	1.880	1.880
Rpim	0.047	0.860
Number of reflections	87087	12470
<I/σ(I)>	8.7
Completeness [%]	97.4
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05 M KCl, 0.1 M HEPES pH 7.0, 12.5% PEG-4000