9DAM
Crystal Structure of a DARPin Fused to the 1TEL Crystallization Chaperone via a Direct Helical Fusion in a 3-Fold Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 65 |
| Unit cell lengths | 83.290, 83.290, 58.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.400 - 1.780 |
| R-factor | 0.2053 |
| Rwork | 0.204 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.020 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 1.844 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.152 | 5.909 |
| Rmeas | 0.155 | 6.060 |
| Rpim | 0.034 | 1.332 |
| Number of reflections | 22160 | 1639 |
| <I/σ(I)> | 14.13 | 0.6 |
| Completeness [%] | 97.2 | 75.01 |
| Redundancy | 21 | 20.2 |
| CC(1/2) | 0.999 | 0.335 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6 |
