9D9J

T4 Lysozyme A73H/G77H co-crystallized with Cu(II)-NTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 32 2 1
Unit cell lengths	59.038, 59.038, 95.645
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.210 - 1.450
R-factor	0.1573
Rwork	0.156
R-free	0.17650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.647
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.210	1.490
High resolution limit [Å]	1.450	1.450
Number of reflections	34720	2425
<I/σ(I)>	14	2.6
Completeness [%]	99.7
Redundancy	11
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	Hanging drop: 2.5 uL of 330 uM protein with 2475 uM Cu(II)-NTA + 2.5 uL mother liquor. Mother liquor: 2.0 M NaH2PO4/K2HPO4, pH 6.8, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol