9D9F
Candida albicans Hsp90 nucleotide binding domain in complex with BRI2311
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-04 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.54189 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 73.874, 73.874, 108.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.560 - 1.780 |
| Rwork | 0.175 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.247 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.77)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.560 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 1.145 |
| Rmeas | 0.061 | 1.317 |
| Rpim | 0.017 | 0.635 |
| Total number of observations | 395944 | 6540 |
| Number of reflections | 31120 | 1651 |
| <I/σ(I)> | 31 | 1.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 12.7 | 4 |
| CC(1/2) | 1.000 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 18% PEG8000, 0.1M Cacodylates pH 6.5, 0.2 M MgAcetate, 20% glycerol |
