9D97
KIR3DL1 - HLA-B*38-YHE complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95368 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.963, 60.047, 63.337 |
| Unit cell angles | 94.96, 95.50, 106.23 |
Refinement procedure
| Resolution | 44.740 - 1.600 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.740 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rpim | 0.052 | 0.486 |
| Number of reflections | 84176 | 8259 |
| <I/σ(I)> | 8.61 | 1.48 |
| Completeness [%] | 96.2 | 95.49 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 0.995 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 15% PEG 3350, 2% Tacsimate, pH 5.0, 0.1 M Na3citrate, pH 5.6 |
