9D91
Crystal structure of L-asparaginase from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-05 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.369, 74.016, 132.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.820 - 1.620 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.842 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.090 | 0.618 |
| Rmeas | 0.099 | 0.660 |
| Rpim | 0.042 | 0.262 |
| Number of reflections | 34185 | 34185 |
| <I/σ(I)> | 15.637 | 2.8 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.1 | 5.7 |
| CC(1/2) | 0.993 | 0.856 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M Lithium Sulfate, 0.1 M Tris HCl pH 7.0, 2 M Ammonium Sulfate |
