Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-04-25 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.351, 59.794, 78.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.040 - 1.970 |
| R-factor | 0.15747 |
| Rwork | 0.155 |
| R-free | 0.19803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.735 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.067 | 0.158 |
| Number of reflections | 18346 | 928 |
| <I/σ(I)> | 28.22 | 12.03 |
| Completeness [%] | 95.1 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M Ammonium sulfate, 5% ethanol |
