9D8O

Q108K:K40L:T51V:T53C:R58Y:Y19W:Q38L:Q4A:T29L mutant of hCRBPII in complex with FR1V in the dark state at pH 3.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	31.031, 35.866, 63.915
Unit cell angles	85.84, 86.52, 65.30

Refinement procedure

Resolution	32.540 - 1.320
Rwork	0.200
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.859
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.540	1.340
High resolution limit [Å]	1.320	1.320
Rmeas	0.049	0.370
Rpim	0.015	0.146
Number of reflections	49318	3896
<I/σ(I)>	84.2
Completeness [%]	84.0
Redundancy	9.8
CC(1/2)	0.930	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8