9D8B

High-resolution crystal structure of Vibrio cholerae NFeoB in the GDP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979361
Spacegroup name	P 1
Unit cell lengths	54.046, 59.024, 89.481
Unit cell angles	94.39, 92.98, 113.21

Refinement procedure

Resolution	30.830 - 1.820
R-factor	0.205
Rwork	0.203
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.122
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.645	1.849
High resolution limit [Å]	1.818	1.818
Number of reflections	85262	4302
<I/σ(I)>	4.2
Completeness [%]	93.9
Redundancy	1.8
CC(1/2)	0.997	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	28 % (w/v) PEG 4000, 0.1 M bicine (pH 8.4), 0.2 M ammonium acetate, 0.003 M GDP