9D8A
Structure of Rhizopus azygosporus Kinase in complex with GDP and MG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-06 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.417, 66.460, 73.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.17256 |
| Rwork | 0.169 |
| R-free | 0.23727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS (06.2017) |
| Data scaling software | Aimless (0.7.15) |
| Phasing software | PHASER (2.8.8) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.760 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.150 | 4.708 |
| Rmeas | 0.157 | |
| Rpim | 0.044 | 1.388 |
| Number of reflections | 26297 | 1199 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.5 | 12.3 |
| CC(1/2) | 0.998 | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.2 M NH4Cl, 20% (w/v) PEG-3350, PEG 400 |
