9D5I
Crystal structure of the ILK mutant (R225A)/alpha-parvin core complex bound to MgATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-12-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.822, 116.334, 47.412 |
| Unit cell angles | 90.00, 100.79, 90.00 |
Refinement procedure
| Resolution | 40.370 - 1.500 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.830 |
| Number of reflections | 72513 | 3421 |
| <I/σ(I)> | 28.34 | 2.19 |
| Completeness [%] | 97.1 | 92.1 |
| Redundancy | 4.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 277 | 0.1 M Bis-Tris Propane, 17% PEG3350, 5% 1-Propanol, 5 mM MgCl2, and 0.5 mM ATP |
