9D58

Human Dystrophin tandem calponin homology actin-binding domain crystallized in a closed-state conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	59.774, 143.138, 59.862
Unit cell angles	90.00, 102.21, 90.00

Refinement procedure

Resolution	58.510 - 1.940
R-factor	0.2407
Rwork	0.216
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.640
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.510	1.978
High resolution limit [Å]	1.940	1.940
Rmerge	0.104	0.480
Rmeas	0.126	0.580
Rpim	0.069	0.322
Number of reflections	70503	3535
<I/σ(I)>	7.1	2.1
Completeness [%]	97.9
Redundancy	3.1
CC(1/2)	0.995	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-Tris pH 6.7, 30% w/v Polyethylene monomethyl ether (MPEG) 5,000