9D57
iGABASnFR2 fluorescent GABA sensor in complex with GABA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-21 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.999969 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.501, 109.924, 214.309 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 47.390 - 2.600 |
| Rwork | 0.206 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.610 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.740 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.067 | 0.434 |
| Rmeas | 0.092 | 0.591 |
| Rpim | 0.063 | 0.399 |
| Number of reflections | 105803 | 5388 |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 3.6 | 3.8 |
| CC(1/2) | 0.994 | 0.809 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium formate, 20% w/v Polyethylene glycol 3,350 |
