9D34

FIP200 C-terminal CLAW domain (resid. 1490-1594) in complex with phosphorylated TNIP1 FIP200 interacting peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2024-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 43 21 2
Unit cell lengths	51.339, 51.339, 88.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.570 - 1.420
R-factor	0.2037
Rwork	0.202
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.254
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.570	1.471
High resolution limit [Å]	1.420	1.420
Rmerge		1.496
Rpim		0.325
Number of reflections	22984	1889
<I/σ(I)>	21.41
Completeness [%]	96.3	83.99
Redundancy	20
CC(1/2)	0.996	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Morpheus E4 (12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03 M of each ethylene glycol, 0.1 M MES/imidazole pH 6.5)