9D2X
SpiD ETS-domain (168-273) in complex with the DNA sequence d(AATAAAAGGAAGTGGG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920119 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.934, 42.939, 62.534 |
| Unit cell angles | 79.21, 81.53, 76.19 |
Refinement procedure
| Resolution | 32.350 - 2.290 |
| R-factor | 0.2256 |
| Rwork | 0.221 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.863 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.350 | 2.372 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.039 | 0.332 |
| Rmeas | 0.055 | 0.469 |
| Rpim | 0.039 | 0.332 |
| Number of reflections | 15141 | 1543 |
| <I/σ(I)> | 11.02 | 2.19 |
| Completeness [%] | 91.9 | 93.11 |
| Redundancy | 1.7 | 1.7 |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Looped directly from Index HT (Hampton Research) condition F12 from a robot screen - 200 mM NaCl, 100 mM HEPES, pH=7.5, 25% w/v PEG3350 |
