9D2H
Stigmatella aurantica bacteriophytochrome protein 2 (SaBphP2), photosensory core module, investigated at ESRF(EBS) ID29. Dark Structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-02-12 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.690, 83.400, 86.870 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 58.760 - 2.100 |
| R-factor | 0.1954 |
| Rwork | 0.192 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.081 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.000 | 2.150 |
| High resolution limit [Å] | 1.800 | 2.100 |
| Number of reflections | 50696 | 3314 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 486 | |
| CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 289 | 0.085 M Na-citrate, 25.5 % PEG 4000, 15 % glycerol, 3% benzamidine hydrochloride, pH 5.6. 2:3 protein/precipitant ratio |
