9D1V
Human Beta-B2 Crystallin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.491, 82.915, 153.224 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.870 - 2.000 |
| R-factor | 0.2208 |
| Rwork | 0.219 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.218 | 5.630 |
| Rmeas | 0.222 | 5.729 |
| Rpim | 0.042 | 1.057 |
| Total number of observations | 1433206 | 108585 |
| Number of reflections | 52335 | 3774 |
| <I/σ(I)> | 11.5 | 1 |
| Completeness [%] | 99.1 | |
| Redundancy | 27.4 | 28.8 |
| CC(1/2) | 0.999 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 18% PEG 3350, 0.3 M Potassium sodium tartrate tetrahydrate |
