9D13

Tt Pah2 D148N delta helix apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 43 2 2
Unit cell lengths	60.779, 60.779, 159.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.780 - 1.980
R-factor	0.1936
Rwork	0.192
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.002
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.780	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.095	2.157
Rmeas	0.100	2.251
Rpim	0.028	0.636
Number of reflections	21672	2110
<I/σ(I)>	14.78	1.58
Completeness [%]	99.8	99.39
Redundancy	12.2	12.3
CC(1/2)	0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 3000, 0.1 M CHES pH 8.5 - 9.5