9CY9

Biofilm regulatory protein A from Streptococcus mutans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-18
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	38.700, 83.200, 49.400
Unit cell angles	90.00, 99.27, 90.00

Refinement procedure

Resolution	34.710 - 1.840
R-factor	0.1867
Rwork	0.183
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.862
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.840	1.850
Number of reflections	25291	1278
<I/σ(I)>	37.894
Completeness [%]	94.8
Redundancy	5.9
CC(1/2)	0.995	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 10% PEG 6000, 5% MPD