9CY7

Structure of S.pombe Dis3L2 in complex with oligoU RNA substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97936
Spacegroup name	P 41 21 2
Unit cell lengths	122.838, 122.838, 296.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.790 - 3.520
R-factor	0.2838
Rwork	0.282
R-free	0.31030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.639
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487:0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.790	3.640
High resolution limit [Å]	3.510	3.510
Rmerge	0.150
Number of reflections	29105	2820
<I/σ(I)>	17.67
Completeness [%]	99.6
Redundancy	22
CC(1/2)	0.990	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	12% PEG 4000, 100 mM HEPES pH 7.5, and 100 mM Sodium Acetate