9CXV

Crystal structure of Human FN3K bound with ADP and DMF (I)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92011
Spacegroup name	P 21 21 21
Unit cell lengths	52.833, 112.034, 132.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 1.800
R-factor	0.1776
Rwork	0.177
R-free	0.20470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.559
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487:0000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.720	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.056
Number of reflections	73993	7244
<I/σ(I)>	18
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.990	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	-crystallized in 0.5 M ammonium sulfate, 0.1 M tri-sodium citrate, and 1 M lithium sulfate -soaked in 10 mM DMF, 5 mM MgSO4 and 2.5 mM ADP-AlF3 -cryo-protected in 0.9X reservoir solution + 20% ethylene glycol