9CXR
X-ray Crystallographic Structure of the Poly(Hexamethylene Adipamide) (Nylon66) Hydrolase Nyl50 at Room Temperature
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-03-26 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 54.014, 96.643, 105.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.380 - 2.200 |
| R-factor | 0.1605 |
| Rwork | 0.158 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.491 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.380 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.177 | 0.505 |
| Rmeas | 0.201 | 0.572 |
| Rpim | 0.093 | 0.263 |
| Number of reflections | 28405 | 2797 |
| <I/σ(I)> | 9.98 | |
| Completeness [%] | 98.6 | 99.47 |
| Redundancy | 4.4 | |
| CC(1/2) | 0.940 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | Hampton Research PEGRx HT-D2 (Imidazole 0.1 M, Polyethylene glycol 6,000 20.0 w/v) |
