9CXP

Cyan thermostable protein (CTP) 0.75 at pH 7.4

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-27
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	38.880, 77.368, 81.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.350 - 1.700
R-factor	0.1824
Rwork	0.180
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.350	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.062	1.204
Rmeas	0.065	1.298
Rpim	0.019	0.481
Number of reflections	27950	1450
<I/σ(I)>	19.8	1.8
Completeness [%]	99.9	100
Redundancy	11.3	7.1
CC(1/2)	1.000	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	294.15	0.03 M Magnesium Chloride Hexahydrate, 0.1 M Tris HCl pH 7.4, 30% PEG 4000