9CWL

Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella philomiragia (monoclinic P Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-27
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 1 21 1
Unit cell lengths	35.103, 64.118, 35.471
Unit cell angles	90.00, 106.80, 90.00

Refinement procedure

Resolution	33.960 - 1.800
R-factor	0.1905
Rwork	0.189
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.082
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_5396: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.960	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.152	1.696
Rmeas	0.165	1.839
Rpim	0.065	0.707
Total number of observations	92640	5816
Number of reflections	14044	855
<I/σ(I)>	9.8	1.7
Completeness [%]	99.9
Redundancy	6.6	6.8
CC(1/2)	0.993	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Morpheus A9: 20%(v/v) PEG 500 MME, 10%(w/v) PEG 20000, 100 mM Tris/BICINE, pH 8.5, 30 mM MgCl2 and 30 mM CaCl2. FrphA.20731.a.VL2.PB00125 at 13.9 mg/mL. plate 14180 well A9 drop2, Puck: PSL0911 and PSL0912 merged, Cryo: Direct