9CVB
Crystal structure of human ribokinase in complex with K+, Mg2+, and AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.410, 70.930, 90.920 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 55.967 - 1.450 |
| R-factor | 0.1319 |
| Rwork | 0.130 |
| R-free | 0.17630 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.930 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.048 | 0.632 |
| Rmeas | 0.054 | 0.716 |
| Rpim | 0.024 | 0.329 |
| Number of reflections | 101613 | 4997 |
| <I/σ(I)> | 13.9 | 1.8 |
| Completeness [%] | 99.6 | 96.9 |
| Redundancy | 4.8 | 4.3 |
| CC(1/2) | 0.999 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.15 | 0.1 M TrisHCl (pH 8.5), 25% (W/V) PEG 2000 MME |
