Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.072, 122.946, 36.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.530 - 1.530 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.100 | 1.630 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 40347 | 1739 |
| <I/σ(I)> | 12.4 | 1.4 |
| Completeness [%] | 80.6 | 44.1 |
| Redundancy | 4.1 | 3.34 |
| CC(1/2) | 0.999 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M Bis-Tris pH 5.5, 0.2 M magnesium chloride, 25% (w/v) PEG 3,350 |
