Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-28 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.967, 72.644, 66.430 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 25.430 - 1.870 |
| R-factor | 0.264 |
| Rwork | 0.263 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.840 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (21-NOV-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.780 | 2.020 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 19408 | 970 |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 77.6 | 34.4 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.997 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M Bis-Tris pH 5.5, 0.2 M ammonium acetate, 25% (w/v) PEG 3,350 |
