Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.840, 101.490, 66.730 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 43.270 - 1.290 |
| R-factor | 0.157 |
| Rwork | 0.155 |
| R-free | 0.19350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.270 | 1.336 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 1.592 | |
| Number of reflections | 154796 | 15135 |
| <I/σ(I)> | 9.07 | |
| Completeness [%] | 97.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.999 | 0.341 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | MES 5.5, NaCl, PEG3350 |
