Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.950, 101.890, 66.060 |
| Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
| Resolution | 31.410 - 1.280 |
| R-factor | 0.1522 |
| Rwork | 0.150 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.885 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.570 | 1.326 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.077 | 1.398 |
| Number of reflections | 161488 | 16136 |
| <I/σ(I)> | 7.93 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 22-27% PEG3350 + 0.11-0.20M NaCl + 0.10M MES pH 5.5 |
