Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.630, 101.590, 66.340 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 55.540 - 1.420 |
| R-factor | 0.1607 |
| Rwork | 0.158 |
| R-free | 0.20370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.834 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.540 | 1.471 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.090 | |
| Number of reflections | 118161 | 11697 |
| <I/σ(I)> | 7.08 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 18-23% PEG3350 + 0.11-0.2M NaCl + 0.10M Bis Tris pH 5.5 |
