9CSO

16mer self-complementary duplex RNA with s(2)C:G pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-04-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	1.000026
Spacegroup name	H 3 2
Unit cell lengths	41.339, 41.339, 124.146
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.010 - 1.400
R-factor	0.1909
Rwork	0.189
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.048	0.504
Rmeas	0.051	0.548
Rpim	0.018	0.209
Number of reflections	8355	389
<I/σ(I)>	23.4	3.7
Completeness [%]	99.1	95.6
Redundancy	8.6	6.7
CC(1/2)	0.991	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.4 M Sodium citrate tribasic dihydrate, 0.1 M HEPES pH 7.5