9CSC
CtfAB Native Acetoacetate-CoA Transferase protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-16 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 131.421, 131.421, 158.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.360 - 2.000 |
| R-factor | 0.2238 |
| Rwork | 0.222 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.462 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.230 | 2.075 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.136 | 0.642 |
| Rmeas | 0.141 | 0.666 |
| Number of reflections | 93455 | 9111 |
| <I/σ(I)> | 16.23 | 3.73 |
| Completeness [%] | 99.8 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.997 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 100 mM Na Acetate (pH 4.9-5.5), 6-10% PEG 3350 |
