9CS8

Structure of Azospirillum bacterial CARD crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-04-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	38.937, 78.057, 64.266
Unit cell angles	90.00, 90.13, 90.00

Refinement procedure

Resolution	39.030 - 2.400
R-factor	0.2645
Rwork	0.261
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.030	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.112	0.328
Number of reflections	14812	1501
<I/σ(I)>	4.2	2.2
Completeness [%]	99.0
Redundancy	3.5
CC(1/2)	0.977	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	200 mM Sodium acetate, 100 mM Tricine 7.4, 30% PEG-4000