9CRW
Crystal structure of the Candida albicans kinesin-8 proximal tail domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95299 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.960, 104.570, 118.780 |
| Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.490 |
| R-factor | 0.2349 |
| Rwork | 0.234 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 22.804 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.890 | 2.554 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.070 | |
| Rmeas | 0.076 | |
| Rpim | 0.028 | 1.084 |
| Number of reflections | 140148 | 2489 |
| <I/σ(I)> | 11.2 | 0.8 |
| Completeness [%] | 99.2 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 8.5, 18% polyethylene glycol (PEG) 6000 |
