9CRT

Crystal structure of IgG1 FC at natural pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2023-10-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972
Spacegroup name	P 21 21 21
Unit cell lengths	49.638, 80.673, 136.342
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.520 - 2.190
R-factor	0.2275
Rwork	0.222
R-free	0.28250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.520	2.020
High resolution limit [Å]	1.910	1.910
Rmerge	0.151	1.750
Rmeas	0.157	0.900
Rpim	0.043	0.900
Total number of observations	582810
Number of reflections	43157	6190
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	13.5	13.1
CC(1/2)	0.999	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M Hepes pH 7.5 and 20% PEG 20,000