9CRN
Crystal structure of Streptococcus pyogenes TglA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.272, 88.334, 94.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.020 - 1.960 |
R-factor | 0.1906 |
Rwork | 0.189 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.675 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 31441 | 1508 |
<I/σ(I)> | 10.58 | |
Completeness [%] | 99.8 | |
Redundancy | 6.2 | |
CC(1/2) | 0.970 | 0.426 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM Bis-Tris (pH 6.5), 200 mM sodium chloride, 15% PEG 3350 |