9CQT
Human OxyHb (C2 symmetry) obtained using the SPT Labtech chameleon In the presence of 5 mM sodium dithionite under Al's oil
EM specimen
| Vitrification | Yes |
| Instrument | SPOTITON |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.37 |
| Number of Particle | 3643613 |
| Software | cryoSPARC,PHENIX,EPU |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
