9CQN
Human OxyHb (C2 symmetry) obtained using the SPT Labtech chameleon In the presence of 25 uM sodium dithionite under Al's oil
EM specimen
Vitrification | Yes |
Instrument | SPOTITON |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | TFS Selectris X |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.37 |
Number of Particle | 3643613 |
Software | PHENIX,EPU |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |