9CQB
Antibody 1G8 bound to the central conserved domain of RSV G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | .97 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.394, 67.394, 286.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.370 - 2.500 |
R-factor | 0.2286 |
Rwork | 0.226 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.091 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 286.350 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 27297 | 1310 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 100.0 | |
Redundancy | 18.9 | |
CC(1/2) | 0.997 | 0.553 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | .15M Sodium Thiocyanate and 16% PEG 3350 |