9CPM
Thermus thermophilus HB27 laccase (Tth-Lac) mutant with partial deletion of beta-hairpin sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.927, 66.032, 74.057 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 51.170 - 1.600 |
| R-factor | 0.15484 |
| Rwork | 0.153 |
| R-free | 0.18152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.853 |
| Data reduction software | DIALS (3.20.0) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.030 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.184 | 2.330 |
| Rmeas | 0.218 | 2.789 |
| Rpim | 0.116 | 1.515 |
| Number of reflections | 65047 | 3216 |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.9 | 6.7 |
| CC(1/2) | 0.993 | 0.464 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES (pH6.5), 12% PEG 20K |
