9CPE
Structural basis of BAK sequestration by MCL-1 and consequences for apoptosis initiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 124 |
| Detector technology | PIXEL |
| Collection date | 2022-06-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | P 63 |
| Unit cell lengths | 73.319, 73.319, 45.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.470 - 1.490 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.470 | 2.970 |
| High resolution limit [Å] | 1.480 | 2.360 |
| Rmerge | 0.369 | |
| Number of reflections | 22525 | 2759 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 98.0 | |
| Redundancy | 8.1 | |
| CC(1/2) | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 15-25% PEG 3350, 0.1 M sodium acetate, 0.1M HEPES pH 7.5 |
