9COZ

Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella philomiragia (Orthorhombic P Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-14
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	35.272, 62.107, 79.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 1.700
R-factor	0.1865
Rwork	0.184
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_5383: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.930	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.107	2.052
Rmeas	0.112	2.132
Rpim	0.031	0.575
Total number of observations	258287	13912
Number of reflections	19913	1021
<I/σ(I)>	14.1	1.5
Completeness [%]	100.0
Redundancy	13	13.6
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	Morpheus C12: 20% (v/v) Ethylene glycol; 10 % (w/v) PEG 8000; 0.02M D-Glucose; 0.05M Sodium HEPES; 0.05M MOPS (acid) pH 7.5; 0.02M D-Mannose; 0.02M D-Galactose; 0.02M L-Fucose; 0.02M D-Xylose; 0.02M N-Acetyl-D-Glucosamine; 0.2% w/v Lidocaine hydrochloride monohydrate, 0.2% w/v Procaine hydrochloride, 0.2%w/v Proparacaine hydrochloride, 0.2% w/v tetracaine hydrochloride . FrphA.20731.a.VL2.PB00125 at 13.9 mg/mL. plate 14180 well C12 drop2, Puck: PSL0901, Cryo: Direct