9CMR

Saccharomyces cerevisiae Tom1 HECT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	F 41 3 2
Unit cell lengths	298.998, 298.998, 298.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.300 - 3.300
R-factor	0.2294
Rwork	0.228
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.451
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.300	3.600
High resolution limit [Å]	3.300	3.300
Rmerge	0.058	0.048
Number of reflections	17852	3583
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	32
CC(1/2)	0.995	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	PEG, MgSO4, Tris