9CM4
HCV2 Fab bound to selected RNA loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 215.232, 52.210, 133.338 |
| Unit cell angles | 90.00, 124.92, 90.00 |
Refinement procedure
| Resolution | 54.670 - 1.820 |
| R-factor | 0.1607 |
| Rwork | 0.160 |
| R-free | 0.19840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.670 | 1.885 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.365 | 1.199 |
| Rmeas | 0.405 | 1.330 |
| Rpim | 0.173 | 0.571 |
| Number of reflections | 107770 | 10623 |
| <I/σ(I)> | 13.39 | |
| Completeness [%] | 98.2 | 97.72 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.632 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v polyethylene glycol 3,350 |
