9CLF

Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.398, 89.749, 99.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.810 - 2.360
R-factor	0.15889
Rwork	0.156
R-free	0.20778
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.400
High resolution limit [Å]	2.360	6.400	2.360
Rmerge	0.092	0.036	0.558
Rmeas	0.100	0.039	0.606
Rpim	0.039	0.015	0.234
Total number of observations	190378
Number of reflections	29674	1631	1453
<I/σ(I)>	5.8
Completeness [%]	100.0	100	100
Redundancy	6.4	6.3	6.6
CC(1/2)	0.995	0.999	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	296	0.65 M NaCit, 0.1 M HEPES pH 7.0, 60% glycerol, 10 mM DTT