9CLE

Importin alpha isoform 2 with synthetic zero net-charge nuclear localization signal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.431, 89.818, 99.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.880 - 2.340
R-factor	0.15952
Rwork	0.157
R-free	0.20496
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.340	6.350	2.340
Rmerge	0.080	0.029	0.541
Rmeas	0.088	0.032	0.588
Rpim	0.034	0.012	0.227
Total number of observations	195842
Number of reflections	30438	1676	1509
<I/σ(I)>	6.4
Completeness [%]	100.0	100	100
Redundancy	6.4	6.3	6.6
CC(1/2)	0.997	0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	296	0.7 M NaCit, 0.1 M HEPES pH 6.6, 60% glycerol, 10 mM DTT