9CLD

Crystal structure of maltose binding protein (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	43.819, 64.524, 57.640
Unit cell angles	90.00, 101.17, 90.00

Refinement procedure

Resolution	42.990 - 1.580
R-factor	0.1554
Rwork	0.154
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5058: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.990	1.610
High resolution limit [Å]	1.580	1.580
Number of reflections	42986	2129
<I/σ(I)>	10.6	1.7
Completeness [%]	99.5
Redundancy	5
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	30% v/v PEG400, 100 mM sodium acetate (pH 4.6), 100 mM cadmium chloride