9CLC

Crystal structure of maltose binding protein (Apo), mutant Trp10 to 4-Cyanotryptophan

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	43.914, 64.655, 57.732
Unit cell angles	90.00, 101.42, 90.00

Refinement procedure

Resolution	42.560 - 1.480
R-factor	0.1533
Rwork	0.153
R-free	0.16520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5058: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	1.510
High resolution limit [Å]	1.480	1.480
Number of reflections	50774	2463
<I/σ(I)>	10	2.2
Completeness [%]	96.4	94.5
Redundancy	4	4
CC(1/2)	0.995	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	30% v/v PEG400, 100 mM sodium acetate (pH 4.6), 100 mM cadmium chloride